Research & Development

The Agriculture Experiment Station Chemical Laboratories are a rich blend of research endeavors that have involved the analysis of NASA moon rocks by Dr. Charles Gehrke, to the present day subject matters ranging from metabolomic method development, analysis of soybean nodules, nutriceuticals, to the analysis and study of cystic fibrosis.

Analytical / Biomedical

Metabolomics

Li, Y., R.S. Gitan, D.L. Chance, J.K. Waters, and T.P. Mawhinney. "Comparative Recovery Analysis of Conjugated Linoleic Acids (CLAs) Following Different Methylation Protocols", (2018) American Oil Chemists' Society (AOCS) Annual Meeting & Expo, May 6–9, Minneapolis, MN. [Presentation]

Mawhinney, T.P., R.S Gitan, C.E Cheadle, D.L. Chance, J.K. Waters, V.V. Mossine, and Y.Y. Li. "A Unified Preparatory Method for FAMEs and Sterols in Oilseed Oil and In Situ Tissue for Subsequent GLC Analyses", (2016) American Oil Chemists' Society (AOCS) Annual Meeting, Salt Lake City, Utah, May 1-4. [Presentation]

Wang, W., J. M. Lester, A. E. Amorosa, D. L. Chance, V. V. Mossine, and T. P. Mawhinney. (2015) Facile and Efficient Preparation of Tri-component Fluorescent Glycopolymers via RAFT-controlled Polymerization. J.Vis.Exp. e52922. doi:10.3791/52922 http://www.jove.com/video/52922

Sun, D. H., J. K. Waters, and T. P. Mawhinney. (2000) Determination of thirteen common elements in food samples by inductively coupled plasma atomic emission spectrometry: comparison of five digestion methods. JAOAC Int. 83:1218-1224

Chance, D. L., K. O. Gerhardt, and T. P. Mawhinney. (1998) Gas-liquid chromatography-mass spectrometry of hydroxy fatty acids as their methyl esters tert.-butyldimethylsilyl ethers. J.Chromatogr.A 793:91-98. doi:S0021-9673(97)00876-5

Mawhinney, T. P. and D. L. Chance. 1994. Hydrolysis of sialic acids and O-acetylated sialic acids with propionic acid. Anal.Biochem. 223:164-167. doi:S0003-2697(84)71564-8 ;10.1006/abio.1994.1564

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Agriculture

Strodtman, K. N., S. E. Stevenson, J. K. Waters, T. P. Mawhinney, J. J. Thelen, J. C. Polacco, and D. W. Emerich. 2017. The Bacteroid Periplasm in Soybean Nodules Is an Interkingdom Symbiotic Space. Mol.Plant Microbe Interact. 30:997-1008. https://doi.org/10.1094/MPMI-12-16-0264-R

He, Y., L. Chen, Y. Zhou, T. P. Mawhinney, B. Chen, B. H. Kang, B. A. Hauser, and S. Chen. 2011. Functional characterization of Arabidopsis thaliana isopropylmalate dehydrogenases reveals their important roles in gametophyte development. New Phytol. 189:160-175. https://doi.org/10.1111/j.1469-8137.2010.03460.x

Mahmoud, A. A., S. S. Natarajan, J. O. Bennett, T. P. Mawhinney, W. J. Wiebold, and H. B. Krishnan. 2006. Effect of six decades of selective breeding on soybean protein composition and quality: a biochemical and molecular analysis. J.Agric.Food Chem. 54:3916-3922. https://pubs.acs.org/doi/full/10.1021/jf060391m

Krishnan, H. B., J. O. Bennett, W. S. Kim, A. H. Krishnan, and T. P. Mawhinney. 2005. Nitrogen lowers the sulfur amino acid content of soybean (Glycine max [L.] Merr.) by regulating the accumulation of Bowman-Birk protease inhibitor. J.Agric.Food Chem. 53:6347-6354. https://pubs.acs.org/doi/abs/10.1021/jf050510i

Atalay, A., H. E. Garrett, T. P. Mawhinney, and R. J. Mitchell. 1988. Boron fertilization and carbohydrate relations in mycorrhizal and nonmycorrhizal shortleaf pine. Tree Physiol 4:275-280 https://academic.oup.com/treephys/article-abstract/4/3/275/1674441

Riethman, H. C., T. P. Mawhinney, and L. A. Sherman. 1988. Characterization of phycobilisome glycoproteins in the cyanobacterium Anacystis nidulans R2. J.Bacteriol. 170:2433-2440

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Nutraceuticals

Song, H., Y. Lu, Z. Qu, V. V. Mossine, M. B. Martin, J. Hou, J. Cui, B. A. Peculis, T. P. Mawhinney, J. Cheng, C. M. Greenlief, K. Fritsche, F. J. Schmidt, R. B. Walter, D. B. Lubahn, G. Y. Sun, and Z. Gu (2016) Effects of aged garlic extract and FruArg on gene expression and signaling pathways in lipopolysaccharide-activated microglial cells. Sci.Rep. 6:35323. https://www.nature.com/articles/srep35323

Mossine, V. V., P. Chopra, and T. P. Mawhinney (2008) Interaction of tomato lycopene and ketosamine against rat prostate tumorigenesis. Cancer Res. 68:4384-4391. doi:68/11/4384 ;10.1158/0008-5472.CAN-08-0108 http://cancerres.aacrjournals.org/content/68/11/4384

Mossine, V. V. and T. P. Mawhinney (2007) Nalpha-(1-deoxy-D-fructos-1-yl)-L-histidine ("D-Fructose-L-histidine"): a potent copper chelator from tomato powder. J.Agric.Food Chem. 55:10373-10381. doi:10.1021/jf072092i

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Health & Disease

Glinskii, O. V., S. Sud, V. V. Mossine, T. P. Mawhinney, D. C. Anthony, G. V. Glinsky, K. J. Pienta, and V. V. Glinsky (2012) Inhibition of prostate cancer bone metastasis by synthetic TF antigen mimic/galectin-3 inhibitor lactulose-L-leucine. Neoplasia 14:65

Farid, R., F. A. Murdock, Jr., A. Bonnett, T. P. Mawhinney, D. Chance, J. K. Waters, and J. E. Hewett (2012) Quality of intrathecal baclofen from different sources. PM R 4:182-189. doi:S1934-1482(11)01367-0 ;10.1016/j.pmrj.2011.10.013

Huffman, D. M., T. S. Altena, T. P. Mawhinney, and T. R. Thomas (2004) Effect of n-3 fatty acids on free tryptophan and exercise fatigue. Eur.J.Appl.Physiol 92 :584-591. doi:10.1007/s00421-004-1069-6

Konig, P., M. Shatley, C. Levine, and T. P. Mawhinney (1992) Clinical observations of nebulized flunisolide in infants and young children with asthma and bronchopulmonary dysplasia. Pediatr.Pulmonol. 13:209-214

Cystic Fibrosis

Mossine, V. V., D. L. Chance, J. K. Waters, and T. P. Mawhinney (2018). Interaction of Bacterial Phenazines with Colistimethate in Bronchial Epithelial Cells. Antimicrob.Agents Chemother. https://doi.org/10.1128/AAC.02349-17

Mossine, V. V., J. K. Waters, D. L. Chance, and T. P. Mawhinney (2017) Transient Proteotoxicity of Bacterial Virulence Factor Pyocyanin in Renal Tubular Epithelial Cells Induces ER-Related Vacuolation and Can Be Efficiently Modulated by Iron Chelators. Toxicol.Sci. 155:512. https://doi.org/10.1093/toxsci/kfw174

Chance, D. L. and T. P. Mawhinney (2017) Using Negative Staining TEM to Study Structure/Function Relationships of Cystic Fibrosis Host-Adapted Opportunistic Pathogen Pseudomonas aeruginosa. Microsc.Microanal. 2017/08/04:1354-1355. https://doi.org/10.1017/S1431927617007437

Chance, D. L. and T. P. Mawhinney (1996) Disulfated oligosaccharides derived from tracheobronchial mucous glycoproteins of a patient suffering from cystic fibrosis. Carbohydr.Res. 295:157-177. http://dx.doi.org/10.1016/S0008-6215(96)90136-6

Mawhinney, T. P., D. C. Landrum, D. A. Gayer, and G. J. Barbero (1992) Sulfated sialyl-oligosaccharides derived from tracheobronchial mucous glycoproteins of a patient suffering from cystic fibrosis. Carbohydr.Res. 235:179-197. https://doi.org/10.1016/0008-6215(92)80087-H

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